Monteiro P, Barak B, Zhou Y, McRae R, Rodrigues D, Wickersham IR, Feng G. J Physiol. 2018 May 29. doi: 10.1113/JP275936. [Epub ahead of print] Dorsomedial striatum circuitry is involved in goal-directed actions or movements that upon repetition become habits, encoded by dorsolateral striatum. An inability to shift from habits can compromise action-control and prevent […]
Nontoxic, double-deletion-mutant rabies viral vectors for retrograde targeting of projection neurons.
Chatterjee S, Sullivan HA, MacLennan BJ, Xu R, Hou Y, Lavin TK, Lea NE, Michalski JE, Babcock KR, Dietrich S, Matthews GA, Beyeler A, Calhoon GG, Glober G, Whitesell JD, Yao S, Cetin A, Harris JA, Zeng H, Tye KM, Reid RC, Wickersham IR. Nat Neurosci. 2018 Apr;21(4):638-646. Recombinant rabies viral vectors have proven useful […]
The BRAIN Initiative Cell Census Consortium: Lessons Learned toward Generating a Comprehensive Brain Cell Atlas.
Ecker JR, Geschwind DH, Kriegstein AR, Ngai J, Osten P, Polioudakis D, Regev A, Sestan N, Wickersham IR, Zeng H. Neuron. 2017 Nov 1;96(3):542-557. A comprehensive characterization of neuronal cell types, their distributions, and patterns of connectivity is critical for understanding the properties of neural circuits and how they generate behaviors. Here we review the […]
Shin Yim Y, Park A, Berrios J, Lafourcade M, Pascual LM, Soares N, Yeon Kim J, Kim S, Kim H, Waisman A, Littman DR, Wickersham IR, Harnett MT, Huh JR, Choi GB Nature. 2017 Sep 28;549(7673):482-487. Viral infection during pregnancy is correlated with increased frequency of neurodevelopmental disorders, and this is studied in mice prenatally […]
A comprehensive understanding neuronal cell type diversity is an essential guide to selective manipulation and illuminating cell type specific functional contributions toward health and disease. Accordingly, the Brain Initiative Cell Census Network (BICCN) is unifying the efforts of laboratories with unique expertise in anatomy, genetics, electrophysiology, and function to classify neurons and create a common […]
Metazoan organs, including the brain, are composed of various cell types, but the cell type census for even a relatively simple mammalian brain such as the one of the mouse is not available. A whole mouse brain database of molecularly defined cell types organized in a taxonomy and annotated with their precise locations within the […]
Liu K, Kim J, Kim DW, Zhang YS, Bao H, Denaxa M, Lim SA, Kim E, Liu C, Wickersham IR, Pachinis V, Hattar S, Song J, Brown SP, Blackshaw S. Nature. 2017 Aug 31;548(7669):582-587. Multiple populations of wake-promoting neurons have been characterized in mammals, but few sleep-promoting neurons have been identified. Wake-promoting cell types include […]
Yamawaki N, Suter BA, Wickersham IR, Shepherd GM. Cold Spring Harb Protoc. 2016 Oct 3;2016(10) A set of methods is described for channelrhodopsin-2 (ChR2)-based synaptic circuit analysis that combines photostimulation of virally transfected presynaptic neurons’ axons with whole-cell electrophysiological recordings from retrogradely labeled postsynaptic neurons. The approach exploits the preserved photoexcitability of ChR2-expressing axons in […]
Roland B, Jordan R, Sosulski DL, Diodato A, Fukunaga I, Wickersham I, Franks KM, Schaefer AT, Fleischmann A. Elife 5. pii: e16335 (2016). Perturbations in neural circuits can provide mechanistic understanding of the neural correlates of behavior. In M71 transgenic mice with a “monoclonal nose”, glomerular input patterns in the olfactory bulb are massively perturbed […]
We will use high throughput techniques to produce a set of viral vectors that will allow selective expression of transgenes in specific populations of neurons in the brain. Along with many other applications, this will allow optogenetic control, recording, and genomic modification of targeted neuronal populations without the need for production of transgenic or knock-out […]