The BRAIN Initiative Cell Census Consortium: Lessons Learned toward Generating a Comprehensive Brain Cell Atlas.

Ecker JR, Geschwind DH, Kriegstein AR, Ngai J, Osten P, Polioudakis D, Regev A, Sestan N, Wickersham IR, Zeng H. Neuron. 2017 Nov 1;96(3):542-557. A comprehensive characterization of neuronal cell types, their distributions, and patterns of connectivity is critical for understanding the properties of neural circuits and how they generate behaviors. Here we review the […]

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Reversing behavioural abnormalities in mice exposed to maternal inflammation

Shin Yim Y, Park A, Berrios J, Lafourcade M, Pascual LM, Soares N, Yeon Kim J, Kim S, Kim H, Waisman A, Littman DR, Wickersham IR, Harnett MT, Huh JR, Choi GB Nature. 2017 Sep 28;549(7673):482-487. Viral infection during pregnancy is correlated with increased frequency of neurodevelopmental disorders, and this is studied in mice prenatally […]

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Combining Optogenetics and Electrophysiology to Analyze Projection Neuron Circuits

Yamawaki N, Suter BA, Wickersham IR, Shepherd GM. Cold Spring Harb Protoc. 2016 Oct 3;2016(10) A set of methods is described for channelrhodopsin-2 (ChR2)-based synaptic circuit analysis that combines photostimulation of virally transfected presynaptic neurons’ axons with whole-cell electrophysiological recordings from retrogradely labeled postsynaptic neurons. The approach exploits the preserved photoexcitability of ChR2-expressing axons in […]

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Massive normalization of olfactory bulb output in mice with a ‘monoclonal nose’

Roland B, Jordan R, Sosulski DL, Diodato A, Fukunaga I, Wickersham I, Franks KM, Schaefer AT, Fleischmann A. Elife 5. pii: e16335 (2016). Perturbations in neural circuits can provide mechanistic understanding of the neural correlates of behavior. In M71 transgenic mice with a “monoclonal nose”, glomerular input patterns in the olfactory bulb are massively perturbed […]

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National Institutes of Health (NIH), NIMH

We will use high throughput techniques to produce a set of viral vectors that will allow selective expression of transgenes in specific populations of neurons in the brain. Along with many other applications, this will allow optogenetic control, recording, and genomic modification of targeted neuronal populations without the need for production of transgenic or knock-out […]

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BRAIN Initiative award: A platform for high-throughput production of targeting systems for cell-type-specific transgene expression in wild-type animals

Ian Wickersham and co-PI Robert Desimone have been awarded a BRAIN Initiative R01 grant for “A platform for high-throughput production of targeting systems for cell-type-specific transgene expression in wild-type animals”. The project will use high throughput techniques to produce a set of viral vectors that will allow selective expression of transgenes in specific populations of […]

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Postdoc Lei Jin joins the lab

Lei Jin has joined the lab as a postdoctoral fellow, with a bachelor’s in Biotechnology from Hainan University, a master’s in Biology from Tsinghua University, and a Ph.D. in Bioelectronics from RWTH Aachen University. He is supported by a fellowship from the Simons Center for the Social Brain.

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